Coding
kivd

Part:BBa_K273006:Design

Designed by: Karl Brune   Group: iGEM09_Uppsala-Sweden   (2009-10-16)

α-ketoisovalerate decarboxylase (kivd) from Lactococcus lactis | codon optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized using this codon-usage table

Source

Designed by the Uppsala iGEM Team 2009. Synthesized by Bioneer (www.bioneer.com)

References

[http://biocyc.org/META/NEW-IMAGE?object=MONOMER-13247 MetaCyc Enzyme: α-ketoisovalerate decarboxylase]

[http://www.uniprot.org/uniprot/Q684J7 UniProt: α-ketoisovalerate decarboxylase]

[http://www.pnas.org/content/105/52/20653.full.pdf+html Expanding metabolism for biosynthesis of nonnatural alcohols. Kechun Zhanga, Michael R. Sawayab, David S. Eisenbergb, James C. Liao PNAS December 30, 2008 vol. 105 no. 52 20653-20658]

[http://www.pnas.org/content/105/52/20653 Metabolic engineering for advanced biofuels production from Escherichia coli. Shota Atsumi and James C Liao Current Opinion in Biotechnology 2008, 19:414–419. ]